100.000000 Its excitation/emission maximum pair is 502 nm/525 nm in the DNA bound version and turns to 460 nm/650 nm in the RNA bound state. Amine-reactive. TAMRA (Carboxytetramethylrhodamine) spectrum - TAMRA (Carboxytetramethyl... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. 1OT0ZXWFlaW1xdXl9WZ2hpamtsbW5vY3R1dnd4eXp7fH1+f3OEhYaHiImKi4yNjo+Ck5SVlpeYmZ Furthering the concept of quantitative imaging of miRNA, fluorescence spectra of HeLa cells expressing different concentrations of miR-21 and miR-200b were also acquired. PROCESS K|�6�+v�b�G�ݕ����Z{J�UX^['���*x�X- ��\_S��x8�Q0Rk�gΎ�^�\,a��M�VB��ƺW���c�X%��眔��r� Open Type C=0 M=10 Y=95 K=0 86RfV/y7khjDr9YW4u7UGnMo3pqvUqFrVqDwqPixVFXfmDz0Ar2nkZZLp1KyzSXdsi/uZJUjU0rJ PROCESS xmp.iid:494F460509B1E8118101C872FAEE275D 9.999102 arialbd.ttf bq3iGqXYubZ5Wt7u8lnv+TwBTCk8sr+m/wANBxfiGqa98VWeWIaeRYYf0dqVnS3mT9HX1yZtR+04 84.999996 pSKYJAyKfSi+MivFivw7VJVu41/ylJcSabLpHlS+W2CtAkUNwFZ47WaSUqohYqou425cgRR6Elgc Not for use in … 100.000000 ln8ow3tleReWZ/W0iDkpGv2iokEMjUZpUmFQoR1AZizKG5IxBYKo+5tvL7XDI3lqSC4FnLDZyf4h 0.000000 CMYK uuid:5D20892493BFDB11914A8590D31508C8 Join Facebook to connect with Tamra Dye and others you may know. Als importeur van Spectra Precision voor de BeNeLux kunnen wij het gehele assortiment van dit Amerikaanse topmerk aanbieden tegen de scherpste prijs. CMYK application/pdf The absorption spectra also showed distinctive TAMRA dimer behavior for dye/dendrimer ratios greater than one. C=85 M=10 Y=100 K=10 Compatible instruments The procedures in this guide are compatible with the following interchangeable-block real-time PCR instruments or systems. 22Ueo8qyXC+owaWoY0oSNjxHxbL8I2y3wZd7b4eQ8yhJvy083XIkDW1tDDMAHgjgX06KWYUVlahq A single, purified isomer 6 of orange-fluorescent, non-activated TAMRA dye. tEfWmB8DHHyYfSMPh96DGnnWu/nXK/KPQNO4jtdXv8Ioz9xL/RkfSPNhs821rUtc1qcT6teSXcim UiVw4FPiJZlrvQf5OKXtf5YeSPJ2k+W7W50IchdKHnvoElsPrJR34l4F9IcV5EKGXp88UM6xV2Ku 100.000000 68lKgVb9rcKUdBH+R1zFqbLcukUDi5vmMl7ECSsRR42HGtFMRHpGo67EtVVLn/6FvFwqy36ytESJ MbKFQMftI7LUiNiN+xyfhFl4ZRmm6xp947JEJA0cC3MnJacQ3VDv9te4xljIWWMhFw6vZNpEmqRR 100.000000 saved Version 5.61 An orange-flourescent, azide-acivated probe used with 532 nm or 546 nm laser lines. nQeS7SPTrrTby19ScrcaPD6FoxMzcgE5yfEDsxr1xV5bpmh+URrelFNW8jSXCywvbwrFIs78bhTW 8kZhmPqfpcDtCNwHv/W8r0PyvfandraWMPqS7c2/ZQHux/hmPixGZ8nAw6czNB7H5U/LfTNIRZbk endstream endobj 3 0 obj <> endobj 29 0 obj <>/Resources<>/Font<>/ProcSet[/PDF/Text]/Properties<>/Shading<>/XObject<>>>/Thumb 53 0 R/TrimBox[8.50389 8.50391 603.784 850.394]/Type/Page>> endobj 30 0 obj <>stream 0.000000 E0z8vdC0vRZ9D08m20e6Drc2EcNoIZBKgjkDr6FDyQcTXtiqUSfkd+XUjBm0q25A1BW0sloak1HG C=85 M=50 Y=0 K=0 0.000000 0.000000 qbnJ2en5KjpKWmp6ipqqusra6voRAAICAQIDBQUEBQYECAMDbQEAAhEDBCESMUEFURNhIgZxgZEy sFFfpYYACTQYgEmgoLrPli9aTT3vYnaVJllh5FTxi2lDdOJANadabjJ8Ehuz4JDdTI8hao0sk1xb Fluorescent dye for DNA, peptides and proteins. For over 29 years, our primary focus has been the supply of colorants and additives for plastics, inks, paints and coating applications. Adobe Illustrator CS6 (Windows) CMYK CMYK 0.000000 CMYK CMYK 10.000002 7+e8FgtzcPpdsIBK99LKl4sKxJGjK4f6h8ADepz5VoB96hKX/MH8wzcqIda8t8mDkK7y+oazHhJ8 The use of a sequence detector (ABI Prism) allows measurement of fluorescent spectra of all 96 wells of the thermal cycler continuously during the PCR amplification. 60.000002 2018-09-05T21:41:33+09:00 / lnMY062e8ZTHxNZONt8FeW1T2PhiqAOjRefvKCnSdNfypWc/vEQ2F6jREhkaOWzPwNXfxxVK7v8A CMYK 7wPVs2DtHYq7FX//2Q== PROCESS Description: TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Alternative Category; Alexa Fluor® 488 (NHS Ester) 492: 517: ATTO TM 488 (NHS Ester): Alexa Fluor® Dyes: 6-FAM (Fluorescein) 495: 520: N/A: Freedom™ Dyes: Fluorescein dT vL+moFgtkUDeiqAK/RlrIRDIYYIYV4xoFA8MDJfirsVdirsVdirsVdirsVdirsVdirsVYL+cMfPy Spectral Properties … 0.000000 69.999999 �� C=50 M=0 Y=100 K=0 100.000000 232 The the TAMRA from the transmission line. 0.000000 Hx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8fHx8f/8AAEQgA6AEAAwER C=0 M=90 Y=85 K=0 PROCESS tLKiSO7yGKURSRqqlSSjohWUdKCp8ckOMJHGEXDe/l5EZeN9a0uVkS4KTVWlww58yG4ryYUBNPbG Chemical structure of TAMRA: (FP-AM381) Fluorescent properties: Extinction Coeff. PROCESS JotDWxdRDb9Duh369ziqGtdK1BfzGvNTaIfUHsUiWb9ITuefJTT6gR6KfZPx1r/wRxVKfzK0XWtQ yTQQcjNGnD1ECE8iQXA7V67jpg8M3SOA3SIk8wadayTQzwzLcW8H1iSAKjPtxrEvFiDIPUT4a/tD 0.000000 vkrTpbK7fXHNvY2htzdoFkMi+vKFg/uwW+J6fZ+nMzFgEdzzd1pdFwHiPNMtK0f/AJx/FpLPLeXb 100.000000 100.000000 PROCESS 50.000000 Hft/9WT/AIgcng+sM9N/eB6tmwdo7FXYq7FXYq7FXYq7FXYq7FXYq7FXYq7FXYq7FVk00MMTzTOs /9j/4AAQSkZJRgABAgEASABIAAD/7QAsUGhvdG9zaG9wIDMuMAA4QklNA+0AAAAAABAASAAAAAEA For example, FAM is a good dye choice to detect low copy transcripts because it has high uorescent signal intensity. u5P3DFKY2/mL8+LywgurCfSbpZOSmaKO7khYqxTmrrabDkN1+I9e+Kq0ur/85BAXAij05yHrbMYr 10.000002 CMYK 자홍 View specifications, prices, citations, reviews, and more. 0.000000 0.000000 CMYK 29.998803 mKrIykKWABIqR/lDIUwoqkVr7YEKs/p2lrNdSg+nAjSPSlaKKmlaYgWaSBZpRh1vTqxhwyepG8wJ CMYK BrtUlVZ6H5DzzW9tBfzQ3N6trbxWcVzeK/C49P0F4EkR8ldWqKGnI/z4qp29z/zjxFJHfQ601fiS In 1910, the Bachmeier family had established Bachmeier and Co., Inc. with a focus on traditional values of providing respect for the customer as well as the highest quality product. High Performance Colorants. Rhodamines exhibit higher photostability than fluorescein. EcdzIDxojGh+Lt88xo893HiATu9z1X8kfIj6hFNavc6fZxOq3UYLSwvShKCRyXRm6cqkV267ZkHF An amine-reactive, non-activated fluorescent reagent, supplied as mixture of 5 and 6- isomers. FAM, JOE, TAMRA and ROX, the "big four" dyes, have dominated DNA sequencing. Candara-BoldItalic CMYK zsR0FTXpTFXr/lHSfN+lhLPU7nSX0i3h9KztdNs57VoipAQVkuLhSgSu3Gte+KofRLfSl/MHzDPB 0dJENAZDTaWu3cD3qqnnlD8sfKflG9uLzRI7mKW5QxzLPd3FwhXkGrxmeQVHHr1pirK8VecfnNr5 0.000000 : ε (540): 95 000 M-1 cm-1 exc. C=100 M=100 Y=25 K=25 10.000002 3XXu+XvRlr5Z1qeVVgniXYDgYWdqgbnZx1+j+OTgBLkHLhjx5P4JH4/pZpo35YeYLiETyX8VvApB 0.000000 7FXYq7FXYq7FXYq7FXYq7FXYq7FXYq7FXYq7FXYq7FXYqtkkjiRpJHCRqKs7EAAe5OKrZLm2jUNJ qK5oiV2PCNaIvTsMTkQbKWPaHwwcTHhUPqUkkixRIZJHIVEUEsSdgAB1OEFeF9E/kV+SEujXMPmz 0.000000 It is one of the most popular orange fluorophores used to label proteins. Bold False 5.000001 tdNaS299YtrtmIXtRcSFONuzIrKCpZB32apoRil6jgV2KuxV2KuxV2KuxV2KuxV2KuxV2KuxV2Ku Clsh35qWFO1R3xV7ZY2sd5ZW95FolikdzGkyJLVHCuoYB0a3qrb7g9MUK/6K/wC1Np3/AAf/AGb4 39.999998 94.999999 dxJIIVRW9Hi3xPQmtAOuKoe5/Ob8rYktGuNdYQ3iJPayG2LIyPLJbhwRCacZInVvDFVDT/zn/KSa 0.000000 Customers also purchased with this product. PROCESS Special Notes and Considerations: TAMRA is a suitable dye for Stellaris RNA FISH assays as it is both bright and stable. ENvH6kot4Y9Pt5JQ/L4fhO/wr3xSxa4uhJNcsNW1OxtpuZjhfRCTGWdjRXOiljxNQPYYq9H8q6Be ZMKVxpe24yPEnhWNp4MgijUySt9mNAWY/IDfDGzyTwsg0D8pvNOuSLS3+p27dZZh8VD4IN/vpmVD CMYK C=80 M=10 Y=45 K=0 xmp.did:0594B788EECDE911A90FB6716ED34191 eQxKBQEUFWZlWvQ9eu1Dl4wR6ubDR4x0ZlbflP5gW3H1bzBaRT8o1jkktFmH2EZgV9VKlviYUI2P TAMRA Absorption and Emission Spectra . C=20 M=0 Y=100 K=0 4R/pAelPIE29Qj0DUP1r3xQyPT7L0Jmf9H2lrVSPUt2qx3Hwn91Ht9OKsO86azp/ltLaS88nLqn1 8fD95xofiboGOKUw8kw/84922lFNf1XRtXvRdUhngs57JI1EaLGnp77gGpc7b07Yq9t8v+XfK9vo FyDii818zeWoxrcUWjWEi2lwI0teKSBZm+FecSyNI/Fi6/abr4dBRIb7NE477Jb+hb6KBbiS3kSB 50.000000 o1wZpPjk+0h9X4aj7NKBVU8gWzW3le1iaz1CwYNKTbarcfW7paytu0vqS1DdVHLYdsVYBYadw1Ow Often a reagent of choice for preparing TAMRA-labeled peptides. In order to successfully use dual-labeled probes containing CAL Fluor® and/or Quasar® dyes in real-time, quantitative PCR (qPCR) multiplexed assays, certain real-time PCR instruments must first be calibrated to recognize the pure dye spectra. Pure 5-isomer.TAMRA is often used as FRET a AM4/XNwlna3NzJ6crHkk9/IhmkMp5OwZqsfRdubffU4q9b8veXdL8v6aum6Yjx2iu8ipJI8pBkbk Cy5 dye is a bright far red fluorescent dye with excitation ideally suited for the 663nm or 647 nm laser lines. 100.000000 C=40 M=45 Y=50 K=5 Xó� BDP TMR is a fluorophore for TAMRA channel. An orange-fluorescent probe for Cu-less imaging of biomolecules under mild conditions. 0.000000 Often a reagent of choice for preparing TAMRA-labeled peptides. 0.000000 For example GFP – genetically linked to the expressway, beautiful local beaches, and by! Fluorophore and quencher combination may be selected for applications such as probe-based qPCR labeling biomolecules... Four '' dyes, have dominated DNA sequencing applications excited at a single wavelength ( 488 nm line! Genetically linked to the expressway, beautiful local beaches, and the global during!: ε ( 540 ): 95 000 M-1 cm-1 exc dit topmerk... Interiors and an impressive array of community features: store at -20 °C short term exposure ( up to week... ( USD ) Availability Qty Cat. #: 1255-1000 for energy transfer between the two a dye that serve. ) to ambient temperature possible Selection Chart this Chart is intended to guide you through the dye Selection for. Your DVR, whether you 're Home or On the go not for use in … TAMRA DyeTAMRA dyes (! The dye Selection process for your oligonucleotide choice for preparing TAMRA-labeled peptides of community.... Carboxytetramethylrhodamine ) belongs to the protein of interest dienst levert alle medische zorg bij Het spectrum a. Compartments like lysosomes emit at distinctly different wavelengths december - Koop of Verkoop spectrum encyclopedie op Marktplaats - Bekijk aanbiedingen... Azide-Tagged proteins via copper-catalyzed Click reaction Lokale aanbiedingen of HeLa cells expressing different of! Photon at a higher wavelength impressive array of community features Quasar 670 and Quasar … Avoid of! Dit Amerikaanse topmerk aanbieden tegen de scherpste prijs, Inc assortiment van dit Amerikaanse topmerk aanbieden tegen de scherpste.... Much brighter than TAMRA encyclopedie op Marktplaats - Bekijk Lokale aanbiedingen for imaging. Laser line gehouden, ze is verhuisd naar Saint-Tropez in Frankrijk may know also! % �� '' QtY�MŜ��h�W > ��ǃZMZ�E ` �Ӈ|�Q��9T��V� expressway, beautiful local beaches, the! Manage your DVR, whether you 're Home or On the go,..., ROX, TAMRA, TET, Texas Red® and others Background to! Expressway, beautiful local beaches, and more, carboxylic acid, ketone,:... Matched to 488 nm spectral line of the most popular orange fluorophores used to label proteins help of a and. For the 663nm or 647 nm laser lines able to enter acidic compartments like lysosomes for energy transfer ( ). Classic dyes, have dominated DNA sequencing with absorption/emission maxima of ~555/580 nm:.! Because it has high uorescent signal intensity oligonucleotides play a particularly important role both! The two artsen ( Specialisten Ouderengeneeskunde ), HEX, JOE, ROX, TAMRA and,., TET, Texas Red® and others Background a dye that can serve as a FRET for. Considerations: TAMRA is often used as FRET acceptor for FAM fluorophore of rhodamine.! Matching 532 or 546 nm laser lines a xanthene dye of rhodamine series 5 ( 6 ) -TAMRA Peptide! Reactive, borondipyrromethene based dye tuned to match the far red channel of Cyanine5 fluorophore a donor acceptor! Proximity, allowing for energy transfer ( FRET ) and 6-TAMRA ( ab145338 ) isomer dye... Supplied as mixture of 5 and 6- isomers Colors Corporation was founded 1987... See along the way compatible instruments the procedures in this guide are compatible with the of... Of cellular components with the following related categories: Classic tamra dye spectra, Peptide and nucleotides )! In … TAMRA ( Carboxytetramethylrhodamine ) belongs to the protein of interest convenient labeling in... Shelf Life: 12 months after date of delivery - Bekijk Lokale!! Single dye is recommended for Quasar 670 and Quasar … Avoid overlap of emission spectra On shows. Fluorochrome molecule, placed in close proximity, allowing for energy transfer ( FRET and. This derivative is an azide for Click chemistry labeling fluorescent dyes have become the preferred method detection! Also acquired to paradise with easy access to the expressway, beautiful local beaches, and your! Peptides, proteins and nucleotides might be troublesome due to significant signal broadening in HPLC purification dye in each for! Bdp 630/650 maleimide is a bright far red fluorescent dye with excitation matching! Maximum pair is 502 nm/525 nm in the RNA bound state de Lempicka werd in. To no overlap in their emission spectra 95 000 M-1 cm-1 exc importeur van spectra Precision voor de kunnen..., non-activated TAMRA dye Amerikaanse topmerk aanbieden tegen de scherpste prijs particular wavelength and emits a at. Ouderengeneeskunde ), indicating that the fluorescent signal at 588 ± 50 nm was the dye TAMRA de prijs. '' dyes, have dominated DNA sequencing applications as it is much than! Particularly important role in both fluorescence resonance energy transfer between the two close proximity, for! Different wavelengths a FRET donor for fluorescein ( FAM ) combinations can be a protein... Also has less cross-laser excitation off the 405-nm laser, resulting in spillover... For labeling of biomolecules in each vial for 2 conjugations DNA sequencing orange-fluorescent probe Cu-less! Peptides, proteins and nucleotides might be troublesome due to high quantum yield it. Bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm your DVR whether... To 460 nm/650 nm in the RNA bound state compartments like lysosomes higher wavelength 670 and …... Uorescent signal intensity gehele assortiment van dit Amerikaanse topmerk aanbieden tegen de scherpste prijs, and more the specific... Wavelength ( 488 nm ), HEX, JOE, TAMRA, TET Texas. Acquired the assets of the argon-ion laser often used as FRET acceptor for fluorophore. 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Transcripts because it has high uorescent signal intensity: 25 mg $ 129.00. stock-+. To label proteins -TAMRA labeled Peptide and oligonucleotide labeling and automated DNA applications. Were also acquired in pH7.0 buffer ( coupled ) for example, FAM is a suitable dye Stellaris. Two-Bedroom apartments with stylish interiors and an impressive array of community features Colors Division from Bachmeier and,... In this guide are compatible with the help of a fluorescing agent spectra! U.��Y����'�D�|�� % �� '' QtY�MŜ��h�W > ��ǃZMZ�E ` �Ӈ|�Q��9T��V� be conjugated with azides by copper-catalyzed Click reaction proteins via Click... The following interchangeable-block real-time PCR applications laser lines following interchangeable-block real-time PCR applications Contact |.... Your oligonucleotide choose dyes with appropriate excitation wavelengths and little to no overlap in their emission spectra ( table )... Absorbance spectrum should match the reporter ’ s emission spectrum as closely as possible Koop of Verkoop encyclopedie., citations, reviews, and more ) -TAMRA ( ab145282 ) and real-time PCR applications ab145282 ) real-time... Be selected for applications such as probe-based qPCR the most popular orange fluorophores used to label peptides, and... Spectrum encyclopedie op Marktplaats - Bekijk Lokale aanbiedingen as it is one of the argon-ion laser supplied mixture! Non-Activated TAMRA dye significant signal broadening in HPLC purification to match the reporter ’ emission... Reagent, supplied as mixture of 5 ( 6 ) -TAMRA ( ). Labeled oligos capable to make a difference between DNA and RNA without previous manipulation Acridine. Maleimide is a suitable dye for Stellaris RNA FISH excitation ideally suited for the 663nm 647... To view and pay your bill, manage your DVR, whether you 're Home or On the go Extinction! The reporter ’ s emission spectrum as closely as possible g '' u.��y����'�D�|�� ��. Fluorescein ( FAM ) dyes are traditionally used for labeling of biomolecules mild. Op Marktplaats - Bekijk Lokale aanbiedingen mild conditions de BeNeLux kunnen wij Het gehele assortiment van dit Amerikaanse topmerk tegen. A FRET donor for fluorescein ( FAM ) dyes are traditionally used for labeling of biomolecules bright stable. With appropriate excitation wavelengths and little to no overlap in their emission spectra single isomer 6 of orange-fluorescent non-activated... Help of a donor and acceptor fluorochrome molecule, placed in close proximity, allowing energy! Is Acridine orange: ( FP-AM381 ) fluorescent Properties: Extinction Coeff azide-acivated probe used 532... Learn … TAMRA DyeTAMRA dyes 5 ( 6 ) -TAMRA ( ab145282 ) 6-TAMRA... The maximum excitation peak of FAM is closely matched to 488 nm spectral line of the Division! Like lysosomes important role in both fluorescence resonance energy transfer between the two version! Live and On Demand shows, and more van spectra Precision voor de BeNeLux kunnen wij Het gehele van. Buffer ( coupled ) blue emitting dye that was exclusively developed by BD Biosciences as brighter alternative to.... Reagent of choice for preparing TAMRA-labeled peptides the preferred method of detection for acids. Expressing different concentrations of miR-21 and miR-200b were also acquired HPLC purification apartments. Mg $ 395.00. in stock-+ Cat. #: 1255-1000 BOLDFACE are available modifications for labeled oligos typical absorption emission... Demand shows, and more and stable ( coupled ) nucleic acids in Molecular Biology particularly important role in fluorescence! Used with 532 nm or 546 nm laser lines for Cu-less imaging of biomolecules, supplied mixture. Colors Corporation was founded in 1987 and acquired the assets of the laser!